Journal: Cell reports
Article Title: DEPDC5-dependent mTORC1 signaling mechanisms are critical for the anti-seizure effects of acute fasting
doi: 10.1016/j.celrep.2022.111278
Figure Lengend Snippet: (A) Fasting protocol for 24 h of 6- to 8-week-old mice randomized to either fast or fed condition. Studies were performed at the same time of day. Separate cohorts were either euthanized for blood and brain collection or administered the chemoconvulsant pentylenetetrazol (PTZ) to induce seizures. (B) Compared with fed mice, those fasted for 24 h had a reduction in seizures after PTZ challenge (65 mg/kg intraperitoneal injection) (n = 25 fed [14 M, 11 F]; 27 fasted mice [14 M, 13 F]; *p = 0.0126, log rank test). (C) Immunoblots from cortical brain lysates and (D) relative quantifications demonstrate a reduction in pSer240/244 S6 and pSer473 AKT after 24-h fasting. Individual symbols represent each independent animal tested with mean ± SD. **p < 0.01, ****p < 0.0001, Student’s t test. (E) After PTZ (65 mg/kg intraperitoneal injection), there was no difference between seizure susceptibility between fed or fasted Depdc5 cc+ mice, which were significantly increased compared with littermate control mice (log rank test, *p < 0.05, **p < 0.01). Six- to 8-week-old mice randomized to either fast or fed condition; n = 20 control fed (8 M, 10 F), 19 control fasted (7 M, 12 F), 19 Depdc5 cc+ fed (8 M, 10 F), and 120 Depdc5 cc+ fasted (9 M, 11 F) littermate mice. (F) Immunoblots and (G) relative quantifications from cortical brain lysates demonstrate a reduction in pSer240/244 S6 after 24-h fasting in only controls; hyperactive mTORC1 in Depdc5 cc+ mouse brains was unchanged by fasting. Individual symbols indicate individual animals for the above measurements, mean ± SD with *p < 0.05, **p < 0.01, ****p < 0.0001. Two-way ANOVA with Tukey’s post hoc analysis.
Article Snippet: Depdc5-sh-GFP , Origene , TL508165.
Techniques: Injection, Western Blot
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![(A) Fasting protocol for 24 h of 6- to 8-week-old mice randomized to either fast or fed condition. Studies were performed at the same time of day. Separate cohorts were either euthanized for blood and brain collection or administered the chemoconvulsant pentylenetetrazol (PTZ) to induce seizures. (B) Compared with fed mice, those fasted for 24 h had a reduction in seizures after PTZ challenge (65 mg/kg intraperitoneal injection) (n = 25 fed [14 M, 11 F]; 27 fasted mice [14 M, 13 F]; *p = 0.0126, log rank test). (C) Immunoblots from cortical brain lysates and (D) relative quantifications demonstrate a reduction in pSer240/244 S6 and pSer473 AKT after 24-h fasting. Individual symbols represent each independent animal tested with mean ± SD. **p < 0.01, ****p < 0.0001, Student’s t test. (E) After PTZ (65 mg/kg intraperitoneal injection), there was no difference between seizure susceptibility between fed or fasted <t>Depdc5</t> cc+ mice, which were significantly increased compared with littermate control mice (log rank test, *p < 0.05, **p < 0.01). Six- to 8-week-old mice randomized to either fast or fed condition; n = 20 control fed (8 M, 10 F), 19 control fasted (7 M, 12 F), 19 Depdc5 cc+ fed (8 M, 10 F), and 120 Depdc5 cc+ fasted (9 M, 11 F) littermate mice. (F) Immunoblots and (G) relative quantifications from cortical brain lysates demonstrate a reduction in pSer240/244 S6 after 24-h fasting in only controls; hyperactive mTORC1 in Depdc5 cc+ mouse brains was unchanged by fasting. Individual symbols indicate individual animals for the above measurements, mean ± SD with *p < 0.05, **p < 0.01, ****p < 0.0001. Two-way ANOVA with Tukey’s post hoc analysis.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8617/pmc09508617/pmc09508617__nihms-1833562-f0002.jpg)
